Table S1 A.

Table 1B.

Table S1.  Cells were sorted to high purity directly from the pancreas lesion of 3­4-wk-old BDC2.5 mice for the CD4+CD25+CD69- population, or the CD25loCD69+ and CD25-CD69-combined populations.  RNA was prepared and hybridized to Affymetrix U74Av2 array GeneChips® as described in the text.  Presented are 96 genes (ESTs not depicted) overexpressed by CD4+CD25+CD69- cells, and 73 genes underexpressed by those cells relative to the CD25loCD69+ and CD25-CD69-combined cell populations.  Genes in common between this list and those identified by McHugh et al. (45) are highlighted in yellow.

Dist CD25- CD69+/-

Dist CD25+ CD69-

Fold chg

LOD

Gene

Alternate gene name

Locus link

Probe name

UP in CD25+

           

41.7

609

14.6

3.57

Klrg1

killer cell lectin-likeR G1

50928

95320_at

32.8

469

14.3

2.21

Gzmb

granzyme B

14939

102877_at

35.7

504

14.1

8

Socs2

suppress of cytokine sig 2

216233

99475_at

43.5

486

11.2

1.21

Ccr5

chemokine (C-C) receptor 5

12774

161968_f_at

14.6

143

9.8

2.28

CD103

integrin, αE

16407

93961_at

26.2

231

8.8

1.55

Il1r2

interleukin 1 receptor, type II

16178

161689_f_at

126.9

1084

8.5

2.12

Lgals1

lectin, galactose binding sol 1

16852

99669_at

112.4

929

8.3

5.57

OX40

tumor necrosis factor rsf4

22163

102681_at

101.2

809

8

3.51

calcyclin

S100 calcium binding protein A6

20200

92770_at

53

397

7.5

4.72

Penk1

preproenkephalin 1

18619

94516_f_at

13.3

82.9

6.3

1.3

Ahr

aryl-hydrocarbon receptor

11622

160495_at

88.5

529

6

2.11

Serpina3g

ser prot inhib., clade A, 3G

20715

102860_at

40.4

239

5.9

3.59

Nrp

neuropilin

18186

95016_at

56

299

5.3

2.11

Ctla4

cytotox T-lymph-assoc. 4

12477

97718_at

305

1572

5.1

2.51

Mela

envelope protein, retroviral

17276

97282_at

36.5

186

5.1

1.66

Mki67

antigen ident. by mAb Ki 67

17345

99457_at

7.18

35.8

5

0.68

Il1rl1

interleukin 1 receptor-like 1

17082

98500_at

90.4

445

4.9

2.35

Anxa2

annexin A2

12306

100569_at

22.1

108

4.9

1.96

Il10

interleukin 10

16153

100319_at

81.4

363

4.5

3.96

Gbp3

guan nucl binding prot 3

55932

103202_at

7.28

32.4

4.4

0.77

Fgl2

fibrinogen-like protein 2

14190

97949_at

52.2

228

4.4

2.92

Tcrg-V2

T cell receptor γ, variable 2

21636

102695_at

25

105

4.2

3.03

Capg

cap prot (actin), gelsolin-like

12332

160106_at

16.8

67.9

4

1.8

Il2ra

interleukin 2 R, α chain

16184

101917_at

60.7

243

4

3.19

S100a4

S100 calcium bind prot A4

20198

99051_at

90.9

353

3.9

3.41

p75 TNFR

tumor necrosis factor rsf1b

21938

94928_at

55.7

213

3.8

1.77

Kdt1

kidney derived trans. 1

16543

102644_at

16

57.7

3.6

1.62

Anxa1

annexin A1

16952

93038_f_at

16.3

57.9

3.5

4.6

Blimp-1

PR dom cont. 1, w/ZNF dom.

12142

92904_at

358.4

1265

3.5

2.41

calpactin

S100 calcium bind prot A10

20194

92539_at

30.6

100

3.3

2.3

Ccr2

chemokine (C-C) R 2

12772

93397_at

95.2

305

3.2

5.54

Cxcr3

chemokine (C-X-C) R 3

12766

94173_at

12.8

39.7

3.1

1.1

Ier5

immediate early response 5

15939

92773_at

93.2

287

3.1

3.37

Dstn

destrin

56431

94492_at

43.9

134

3.1

5.44

Laptm4b

lysosomal-assoc prot. TM 4B

114128

100571_at

218.7

640

2.9

5.32

GITR

tumor necrosis factor rsf18

21936

104662_at

12.7

37.1

2.9

0.96

Tacc3

transforming, acid coiled-coil  3

21335

97238_at

46.9

135

2.9

1.8

Rrm2

ribonucleotide reductase M2

20135

102001_at

163

456

2.8

2.68

Igh-6

Ig heavy chain 6 (IgM)

16019

93584_at

386

1075

2.8

3.25

Crip

cysteine rich intestinal protein

12925

94061_at

114

314

2.8

2.39

Ccnb2

cyclin B2

12442

94294_at

35.7

98.3

2.8

1.12

Top2a

topoisomerase (DNA) II α

21973

99578_at

42.9

118

2.7

1.8

Shyc

selective hybridizing clone

20430

102752_at

31.5

85.4

2.7

1.96

Rin2

Ras and Rab interactor 2

74030

160827_at

1626

4402

2.7

5.59

Sca-1

lymphocyte Ag 6 complex, A

110454

93078_at

64.2

173

2.7

2.44

Hop

homeodomain only protein

74318

96672_at

76

200

2.6

3.13

Cish

cyt. induc. SH2-contain prot.

12700

100022_at

33.6

88.2

2.6

2.92

Cdc2a

cell division cycle 2 hom. A

12534

100128_at

10

26.2

2.6

1.66

Gp49a

glycoprotein 49 A

14727

100325_at

131

339

2.6

1.68

Igk-V8

Ig κ chain variable 8

16123

93086_at

73.9

190

2.6

2.77

Ncf4

neutrophil cytosolic factor 4

17972

103662_at

17

43.6

2.6

3.14

calgizzarin

S100 calcium bind prot. A11

20195

98600_at

298

762

2.6

2.77

Samhd1

SAM domain and HD domain, 1

56045

103080_at

31.5

79.5

2.5

1.24

Xbp1

X-box binding protein 1

22433

94821_at

20.2

50.7

2.5

1.47

Tcrg-V4

T-cell receptor γ, variable 4

21638

102744_at

488

1226

2.5

4.92

Cd2

CD2 antigen

12481

95373_at

10.1

25.3

2.5

3.66

Nfil3

nucl. factor, IL- 3 regulated

18030

102955_at

94.8

237

2.5

1.85

Cks2

CDC28 prot kinase reg. sub. 2

66197

97527_at

10.4

25.5

2.4

3.8

Entpd1

ectonucleoside triP diPhydrolase 1

12495

160774_at

138

337

2.4

3.28

Psen2

presenilin 2

19165

99930_s_at

99.6

241

2.4

1.89

Cst3

cystatin C

13010

99586_at

30.2

72.5

2.4

1.15

Emp1

epithelial membrane protein 1

13730

97426_at

230

552

2.4

2.55

S100a13

S100 calcium bind prot. A13

20196

161121_f_at

228

539

2.4

2.64

Pim1

proviral integration site 1

18712

104533_at

34

80.4

2.4

4.38

Serpinb6

ser prot inhib, clade B, mem 6

20719

96060_at

139

326

2.3

2.8

Glrx1

glutaredoxin 1 (thioltransferase)

93692

95722_at

62.9

147

2.3

1.47

Il18r1

interleukin 18 receptor 1

16182

101144_at

47.8

111

2.3

1.89

Isg20

interferon-stimulated protein

57444

103432_at

582

1348

2.3

1.46

Ly6e

lymphocyte Ag 6 complex, E

17069

101487_f_at

40.5

92.7

2.3

2.55

Xist

inactive X-specific transcripts

213742

99422_at

58

132

2.3

1.32

Calcineurin

prot ppt 3, cat sub, α isoform

19055

95092_at

332.9

758

2.3

2.33

Cd48

CD48 antigen

12506

103089_at

15.6

35.1

2.3

3.68

Tyms

thymidylate synthase

22171

93237_s_at

32.1

72.1

2.2

1.89

Cst7

cystatin F (leukocystatin)

13011

102638_at

49

109

2.2

2.36

Ctsw

cathepsin W

13041

92214_at

104.7

233

2.2

1.92

Vamp5

vesicle-assoc mem. prot. 5

53620

92496_at

51.4

114

2.2

2.89

Ebp

phenylalkylamine Ca2+ antag bind prot

13595

96627_at

211

467

2.2

3.05

H2afz

H2A histone family, member Z

51788

101954_at

51.1

113

2.2

2

Bach

brain acyl-CoA hydrolase

70025

100539_at

19.1

42

2.2

3.35

Icam1

intercellular adhesion molecule

15894

96752_at

54.4

118

2.2

3.39

Ndrl

N-myc downstream reg-like

17990

96596_at

26.3

57.2

2.2

1.17

Gadd45g

growth arrest DNA-damage-ind. 45g

23882

101979_at

185.7

401

2.2

0.85

---

diabetic nephropathy-rel gene 1

NA

100880_at

44.9

96.5

2.2

1.92

Prc1

protein reg. of cytokinesis 1

233406

95032_at

69.4

149.1

2.2

1.21

Wbp5

WW domain binding protein 5

22381

100522_s_at

47.4

101.2

2.1

1.22

Cdc6

cell division cycle 6 hom.

23834

103821_at

46.6

98.7

2.1

2.85

Vamp8

vesicle-assoc. mem. prot. 8

22320

93305_f_at

76.8

162

2.1

3.51

Pttg1

pituitary tumor-transform. 1

30939

101027_s_at

Dist CD25-CD69+/-

Dist CD25+ CD69-

Fold chg

LOD

Gene

Alternate gene name

Locus link

Probe name

DOWN in CD25+

           

96.4

15.4

0.16

4.12

OBF-1

POU domain, cl 2, af 1

18985

93915_at

1370

231

0.17

5.37

Tcf7

transcript fact. 7, T cell specific

21414

97994_at

75.2

15.7

0.21

3.23

Eomes

eomesodermin homolog

13813

103532_at

123.9

27.5

0.22

3.43

Smad1

MAD homolog 1 (Drosophila)

17125

102983_at

245

57.2

0.23

4.82

Itm2a

integral membrane protein 2A

16431

93511_at

125

33.6

0.27

4.52

Nsg2

neuron spec. genefam. mem.2

18197

160413_at

865

253

0.29

2.68

Myb

myeloblastosis oncogene

17863

92644_s_at

66

20.3

0.31

3.39

Dusp6

dual  specificity phosphatase 6

67603

93285_at

58

18.7

0.32

2.92

Actn1

actinin, α 1

109711

104579_r_at

301

102

0.34

2.8

Evl

Ena-vasodilator stim. phosphoprot.

14026

160667_at

280

95.9

0.34

3.04

Ugcg

UDP-gluc. cer. glucosyltransferase

22234

96623_at

140

48.1

0.34

2.7

Bcl6

B cell leukemia/lymphoma 6

12053

103015_at

423

149

0.35

3.77

Rnf19

ring finger protein 19

30945

100509_at

993

360

0.36

2.07

Ncl

nucleolin

17975

100144_at

317

121

0.38

3.44

Cd28

CD28 antigen

12487

102237_at

208

79.2

0.38

2.39

Stk38

serine/threonine kinase 38

106504

94460_at

180

69.4

0.39

1.82

Ets1

E26 av. Leuk. oncogene 1, 5' dom

23871

94332_at

508

203

0.4

3.12

Satb1

special AT-rich sequence bind prot.1

20230

99665_at

62.3

25.6

0.41

2.39

Tex292

testis expressed gene 292

21771

102315_at

61.3

25.3

0.41

3.17

Ubqln1

ubiquilin 1

56085

99575_at

56.1

23.6

0.42

2.4

Ccni

cyclin I

12453

94820_r_at

171

72.3

0.42

2.35

Cct8

chaperonin subunit 8 (Θ)

12469

160102_at

34.3

14.7

0.43

0.82

Dby

DEAD box polypeptide, Y chr

26900

103842_at

348

151

0.43

3.22

Bzw2

basic leu zipper and W2 dom. 2

66912

95462_at

892

387

0.43

2.37

Gtf2i

general transcription factor II I

14886

94295_at

759

329

0.43

1.8

Actb

actin, ß, cytoplasmic

11461

M12481_5_at

100

44.4

0.44

1.43

Sec13l

sec13-like protein

72124

160758_at

168

74.8

0.45

2.6

Calcineurin

prot. ppt 3, reg sub B, α isoform

19058

99183_at

698

314.2

0.45

2.14

Eif4a2

euk. transl. initiation factor 4A2

13682

93089_at

135

61.1

0.45

1.72

Fnbp4

formin binding protein 4

55935

101422_at

377

172

0.46

2.48

Fbl

fibrillarin

14113

160503_at

305

142

0.47

1.54

Max

Max protein

17187

99095_at

41

19.2

0.47

1.43

Cyb5r1

cytochrome b5 reductase 1

72017

103922_f_at

96.4

45.5

0.47

1.55

Il17r

interleukin 17 receptor

16172

99991_at

64.2

30.4

0.47

1.85

Hivep2

HIV type I enhancer bind prot.2

15273

92484_at

44.9

21.3

0.48

2

Atp6ip2

ATPase, H+ transp, lys inter prot1

70495

160202_at

149

70.9

0.48

2.36

Rrs1

RRS1 ribosome biogenesis reg.

59014

96778_at

67.4

32.2

0.48

2.74

Snrpd3

small nucl. ribonucleoprot. D3

67332

98077_at

35.5

17

0.48

2.27

Rcbtb1

reg chrom cond. (RCC1),BTB (POZ)

71330

160959_at

283

136

0.48

2.92

Laptm4a

lysosomal-assoc. prot. transm. 4A

17775

96876_at

82.9

39.9

0.48

2.59

Klhdc2

kelch domain containing 2

69554

95622_at

136

66.7

0.49

2.26

Siat1

sialyltransferase 1

20440

94432_at

1512

746

0.49

3.82

Npm1

nucleophosmin 1

18148

101634_at

1482

732

0.49

3.23

Smc4l1

SMC4 struc maint chrom 4-like 1

70099

101906_at

189

93.5

0.49

0.89

Fos

FBJ osteosarcoma oncogene

14281

160901_at

81.9

40.7

0.5

2.35

Rab2

RAB2, RAS onc. family

59021

94031_at

129

63.9

0.5

1.72

Ssbp2

 ssDNA bind. prot.2

66970

103504_at

165

82.3

0.5

2.72

Paip2

Pabp-interacting protein 2

67869

95743_at

471

235

0.5

2.35

Arl6ip5

ADP-ribosyl.-like fac6 interact. prot5

65106

97424_at