Table S4.  Isotypes of Antichromatin Antibodies

Recipient mouse

Day

CA30 cells received

Antichromatin (serum)

IgM

IgG1

IgG2a

IgG2b

IgG3

IgA

κTg

28

107

+

++

+++

+

+

-

+

+

++

-

(+)

-

130

107

(+)

+

+++

+++

+

-

(+)

+

+

+

-

-

(+)

+

++++

+

+

-

(+)

+

+

+

++

-

(+)

+

++++

+

+++

-

A/J 1

28

107

(+)

-

(+)

-

(+)

(+)

130

(+)

-

(+)

-

-

-

Autoimmune MRL lpr/lpr control

++

++++

++++

++

++++

+

Control Ab 3H9/Vκ4

(+)

-

-

+++

-

-

1A/J mice were also immunized with 30 µg of 894 peptide i.p. at time of transfer.

Isotypes of antichromatin Ab in the sera of recipient mice. Plus signs indicate strength of signal on an arbitrary scale with + corresponding to an OD that was less than twofold above background level at a serum dilution of 1:50 and ++++ corresponding to an OD that exceeded the linear range of the assay at a dilution of 1:200.